Does organic pollution affect E.Coli growth Essay

Does organic pollution affect E.Coli growth - Essay Example The paper tells that biological analysis of water to detect and quantify water pollution is a non-sensitive process. Microbes such as E.coli, which is a fecal contaminant, are able to survive the effects of water pollution through genetic adaptation. The changes in cellular metabolism that take place during this adaptation can be detected at a sensitive level, such as the quantity of E.coli in the water, or the amount of stress proteins being synthesized by the bacteria. Therefore, E.coli can act as a bio-indicator of water pollution. My research question and experiments were designed to study what effects take place on the growth and protein synthesis of E.coli when the bacteria is exposed to environmental stress. The growth of E.coli from lakewater was also compared with that of genetically engineered E.coli DH-5, to determine whether plasmid-mediated mutations assist in the survival of lakewater E.coli under environmental stress. My results showed that DH-5 was inhibited by the po llutant benzene and by high and low pH and temperatures. E.coli from lakewater survived in a greater quantity under these stressors compared t the genetically engineered strain DH-5. Also, the growth of E.coli from lakewater was inhibited to a small extent by the stress of temperature and pH change until a limit. Beyond these limits in the conditions, E.coli could not grow at all. Thus, the adaptation of E.coli to pollution is a characteristic with limitations. In conclusion, the changes in the growth and protein synthesis of E.coli under stress makes it suitable as a bio-indicator of water pollution. Further studies are needed to examine the usefulness of detecting synthesis of specific stress proteins by E.coli as sensitive bio-markers of water pollution. Table of Contents Interest in Topic 5 Introduction 6 Methods 8 Results 12 Discussion, Conclusions and Limitations 13, 14 Images 15 Bibliography 21 Interest in Topic My selection of my research topic is based on my interest in con ducting biological research, my concerns regarding environmental pollution, and my ambition to reduce the consequences of pollution through the results of my research work. I have been highly concerned regarding the many severely polluted water sources in my environment that I have observed. Various unchecked and unregulated commercial and industrial activities near rivers and lakes have released harmful substances into the water, destroying the ecology of these areas. I learned that to prevent and correct these changes, monitoring of the water quality and changes taking place in the aquatic environment are necessary. I have always been fascinated by the science of how some microorganisms work to solve the problem of pollution by producing signals and changes. I was also interested in learning how biological studies are used to derive information and understanding of the many mechanisms happening in our surroundings. After background reading, I developed an interest towards solving the problem of effective biological water analysis, which would